Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp HEPES, Free Acid, 25 KG
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Teknova 1M MES, pH 5.76. 1000mL, Sterile 2 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Electron Microscopy Sciences Mops, Biological Buffer, 6.5-7.9 pH 100 GR
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 6.5-7.9
Bioworld ADA Buffer, 0.2M, pH 6.0, 100 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration50KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
BLD PHARMATECH CO LIMITED Tris(perfluorophenyl)borane, 1109-15-5, Purity: 97%, 10g
Tris(perfluorophenyl)borane, 1109-15-5, Purity: 97%, 10g
Boston Bioproducts Inc Tris-Acetate Buffer (0.2 M, pH 7.8) - 500ml
Tris-Acetate Buffer (0 2 M pH 7 8) - 500ml (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
![eMolecules tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 467454-51-9 | MFCD24466941 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158706.JPG-250.jpg)
eMolecules tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 467454-51-9 | MFCD24466941 | 1g
Pharmablock | tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | 1g | 586155718 | PB07890 | | 467454-51-9 | MFCD24466941 | 213.277 | C11H19NO3
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Bioworld TBE Buffer, Quick-Mix Pack (25X), 2 PK(s)
Product DescriptionTBE (Tris-Borate-EDTA) quick-mix pack prepares 1L of 25X concentrate. Each pack contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Alkali Scientific CellPro™ Tris Glycine SDS Buffer 10X, pH8.3, 1L x 12
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Seracare Life Sciences Inc 10X TRIS BUFFERED SALINE (TBS)
10X Tris-buffered Saline (TBS) is used as a general wash buffer in immunoassay applications.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4) - 4x1L
Tris Buffered Saline-Tween TBST (10X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)