Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline liquid, sterile-filtered, DPBS Modified, without calcium, without magnesium, suitable for cell culture | MFCD00131855 | 1000ML
Dulbecco's Phosphate Buffered Saline liquid, sterile-filtered, DPBS Modified, without calcium, without magnesium, suitable for cell culture | MFCD00131855 | 1000ML
Sigma Aldrich Fine Chemicals Biosciences Trizma hydrochloride, 1185-53-1, MFCD00012590, 500 g
Linear Formula: NH2C(CH2OH)3 · HCl, Molecular Weight: 157.60, BioPerformance Certified, cell culture tested, ≥99.0% (titration), Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride.
Bioworld HEPES, 1M, pH 7.4, Sterile, 250 mL
HEPES BufferAn N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5 Technical Information:Biotechnology GradeDNase(P/F): NoneProtease(P/F): NoneRNase(P/F): NoneAppearance: LiquidBoiling Point : 210-215 °CDensity : 1.33 g/cm3 (Predicted)Melting Point: 234 °C (dec.)PH Value: 7.4RTECS Number: TL6809000HS Code:2933.59.95
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Sigma Aldrich Fine Chemicals Biosciences Tris-EDTA Buffer | CAS No.:Not specified| MDL No.: MFCD00236359 | 1 L
Tris-EDTA buffer | Purity: 100% | M. W.: Not applicable | CAS No.: Not Specified| MDL No.: MFCD00236359 | 1L
Apexbio Technology LLC One-step TUNEL FITC Apoptosis Detection Kit 20rxns
One-step TUNEL FITC Apoptosis Detection Kit can be used to detect nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases become activated cleaving genomic DNA between nucleosomes into 180-200 bp fragments or multiples thereof The resulting 3 -OH DNA ends are labeled with tagged dUTP through the catalytic action of Terminal Deoxynucleotidyl Transferase (TdT) allowing detection via fluorescence microscopy or flow cytometry with excitation/emission wavelengths at Ex/Em 492 nm/517 nm This kit has broad application and can be used to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 200X in DMSO) 100x1ml
When extracting secreted proteins from culture medium in vitro protein production ceases and degradation increases Cell crude extracts contain many endogenous enzymes such as phosphatases and proteases capable of degrading proteins in the extracts The best way to improve the yield of intact proteins is to add inhibitors targeting these known enzymes The Protease Inhibitor Cocktail is used in tissue culture media to enhance protein stability It functions by inhibiting proteases responsible for the degradation of both phosphorylated and non-phosphorylated protein substrates This cocktail is provided as a supplement for tissue culture media to prevent degradation of secreted proteins This Protease Inhibitor Cocktail has been optimized and tested specifically for tissue culture media This protease inhibitor cocktail is provided in DMSO as a ready-to-use solution
Teknova 20X HBS-EP+ Buffer, pH 7.6.1000mL, Sterile.
3M Sodium Chloride, 200mM HEPES Free Acid,60mM EDTA, 1.0% Tween-20
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Rockland Immunochemicals Normal Saline - MB-049-1000
This solution is commonly referred to as physiological saline or isotonic saline.
Zymo Research Corporation DNA/RNA Wash Buffer (Concentrate), 24 ml
DNA/RNA Wash Buffer (Concentrate) (24 ml). For use in Quick-DNA™/RNA purification kits. Sold individually or as part of a nucleic acid purification kit.
Thermo Scientific Production Chemicals and Services TRIS HYDROCHLORIDE 12KG
Avantor: J.T.Baker: TRIS Hydrochloride: Biotech Reagent.12KG/Poly Pail/. 1185-53-1
Teknova 20X PBS Solution, pH 7.2 (at 1X). 10L, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.