Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc TBS HCl (10X), pH 7.2, 500 mL
Tris HCl (10X), pH 7.2 is a Buffered Saline Solution used in procedures for the isolation of DNA.
Filtered through a 0.22 µm membrane
DNase, RNase, and Protease tested
Applications: Tris buffered saline is useful for the following: as a biological diluent buffer, as a buffer for antibodies, as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste Product and/or packaging is safer to human and environmental health
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-25.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-50.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Boston Bioproducts Inc HEPES Buffered Saline (5X, pH 7.4) - 500ml
HEPES Buffered Saline (5X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (20X, with 1% Tween-20, pH 7.4) - 4x1L
Phosphate Buffered Saline-Tween PBST (20X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4, RNase Free) - 250ml
Tris-HCl Buffer (1 M pH 7 4 RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 500ml
MES Buffer (1 M pH 5 0) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5) - 2x1L
Bioprocess Grade - HEPES Buffer (1 M pH 7 5) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc PIPES Buffer (1 M, pH 7.0) - 500ml
PIPES Buffer (1 M pH 7 0) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bis-Tris Transfer Buffer (20X) - 1L
Bis-Tris Transfer Buffer (20X) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc TAPS Buffer (1 M, pH 8.5) - 100ml
TAPS Buffer (1 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
National Diagnostics 1 liter size, TAE Buffer (50X) 0.2 micron filtration
Buffer; National Diagnostics; TAE (50X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Certified RNase and DNase free; Size: 1L
STEMCELL Technologies RoboSep™ Human CD11b Positive Selection and Depletion Kit, For processing: 1 x 10^9 cells
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips